MonoRab™抗VHH納米抗體
駝科動物外周血中存在一種天然缺失輕鏈的重鏈抗體 HCAbs （heavy-chain antibodies )（圖 1）。這種異于傳統雙鏈抗體的重鏈抗體，其抗原結合位點僅由重鏈單鏈的可變區組成，克隆其可變區得到只由一個重鏈可變區組成的單域抗體即為納米抗體（sdAbs或VHHs）。納米抗體VHHs的結構特殊，克服了傳統抗體的開發周期長，穩定性較低，保存條件苛刻等缺陷，逐漸成為新一代治療性生物醫藥與臨床診斷試劑中的新興力量。
基于GenScript專有的雜交瘤細胞技術的 MonoRab™技術平臺，自主研發生產出的抗納米抗體系列產品，為研究人員鑒定目標特異性的納米抗體提供了高性能的二抗工具。
MonoRab™ 抗VHH納米抗體的應用
- VHH納米抗體的篩選和效價測定
- 篩選外周血單個核細胞(PBMC)中特異性表達重鏈抗體的B淋巴細胞
- 胞外域為VHH納米抗體的CAR-T細胞的表達評估及分選
- VHH納米抗體的純化
Anti-Humanized VHH
Anti-Camelid VHH
Anti-Camelid VHH Cocktail
Anti-Camelid VHH Affinity Resin
| Product Type |
Species Specificity |
Unconjugated | Conjugated | ||||||
|---|---|---|---|---|---|---|---|---|---|
| HRP | Biotin | iFluor 488 |
iFluor 555 |
iFluor 647 |
PE | FITC | |||
| Anti-Camelid VHH, mAb |
Llama, Camel, Alpaca |
A01860 | A01861 | A01995 | A01862 | A01863 | A01994 | ||
| Anti-Humanized VHH, mAb |
Humanized, Llama, Camel, Alpaca |
A02165 | A02167 | A02166 | A02169 | A02170 | |||
| Anti-Camelid VHH, mAb Cocktail |
Llama, Camel, Alpaca |
A02014 | A02016 | A02015 | A02021 | A02020 | A02019 | A02018 | A02017 |
Binding Compatibility with Humanized VHHs
Binding compatibility comparison of MonoRab? Rabbit Anti-Humanized VHH Antibody, mAb (Cat. No? A02165 ), MonoRab? Rabbit Anti-Camelid VHH Antibody, mAb (Cat. No? A01860 ), and MonoRab? Rabbit Anti-Camelid VHH Cocktail (Cat. No? A02014 ) with humanized VHHs. The performance of A02165 is the best. It is ideal to be used for humanized VHH antibody detection.
FACS
圖2. 使用不同濃度的 MonoRab? Rabbit Anti-Camelid VHH Cocktail [PE] （GenScript, A02018）通過流式細胞術鑒定CAR-T細胞
隨著CAR-T療法的不斷發展，VHH在構建CAR中的使用也越來越頻繁。該抗體與基于VHH納米抗體的Jurkat-CAR細胞表現出優異的結合活性，建議在CAR-T細胞驗證和鑒定中使用該抗體
MACS
圖3. MonoRab? Rabbit Anti-Camelid VHH Cocktail [Biotin] （GenScript, A02015）在CART細胞磁分離上的應用
實驗采用初始T細胞作為CAR陰性的細胞對照，以9.43% CAR陽性T細胞與初始T細胞混合作為陽性待分離樣品，通過生物素標記的抗體 MonoRab? Rabbit Anti-Camelid VHH Cocktail [Biotin]（GenScript, A02015） 和抗生物素的磁珠進行CART細胞的磁分離，將得到的細胞進行流式細胞分析，以驗證磁分離的效果。如圖所示，經過磁分離后，CAR陽性細胞的比例從預混的9.43%提升至分離后的88.9%，數據表明該抗體可以很好的應用于CART細胞的磁分離。
Sandwich ELISA Application
Standard curve of VHH Sandwich ELISA. The VHH Sandwich ELISA assay is developed by using MonoRab? Rabbit Anti-Camelid VHH Antibody, mAb (GenScript, A01860) and MonoRab? Rabbit Anti-Camelid VHH Cocktail [HRP] (GenScript, A02016) as the capture and detection antibodies, respectively.
In this ELISA assay, the detection antibody was labeled with horseradish peroxidase (HRP), if you needs an unconjugated antibody you can choose MonoRab? Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014). GenScript can provide customized conjugation services for this product per the customer's request.
The sensitivity of detecting VHH is up to 39 pg/ml.
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